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Dissertation Research
Location
Georgia Tech
Date
September 2017 - July 2020
Role
Doctoral researcher
Project type
Phenotypic biological assay
Link
The goal of this project was to develop a novel in vitro assay that mimics fibroblast-mediated remodeling of the provisional fibrin matrix. An aqueous two-phase printing technique was established to enable generation of microscale fibroblast-laden fibrin gels which resemble the early stages of wound healing. Live-cell imaging provided time-course brightfield micrographs that were analyzed through automated image processing protocols for high- throughput evaluation of different stages of remodeling.
By investigating the cumulative effects of fibrinolysis and collagen deposition on fibroblastic remodeling of fibrin, this assay may provide a new resource for advancing understanding of fibrosis pathogenesis and for evaluating potential anti-fibrosis therapeutics.
